Immuno-mass spectrometry imaging (iMSI) uses antibodies conjugated to metal chelating polymers (MCPs) and laser ablation-inductively coupled plasma-mass spectrometry to image and quantify biomolecules within tissue samples [1]. Current challenges with MCPs and antibody conjugate systems involve reproducibility and quantification between tissue samples. To conjugate MCPs to antibodies, disulfide groups within antibodies are utilised to form thiols (by reduction) that can react with maleimide-functionalised MCPs. However, disulfide groups are also responsible for the integral structure of antibodies, hence this method is prone to issues if too many disulfide groups are reduced and the antibody structure is compromised. Furthermore, the formation of multiple thiol units allows for the conjugation of several MCPs, which results in antibody metal conjugates of various metal content. The work presented here includes the synthesis of MCPs, conjugation of MCPs to antibodies, and iMSI data collection that shows improved homogeneity of the MCP-antibody system. The results and future outlook from this work address important issues with antibody-MCP conjugates that affect quantification and reproducibility using iMSI.