Lipid droplets (LDs) are dynamic organelles that are critical for a range of biological functions including lipid storage, hormone production and energy metabolism. The imaging of LD’s can be achieved using commercially available dyes such as BODIPY 493/503 and Nile Red, however these suffer from limitations pertaining to toxicity, off-target staining, photobleaching and storage requirements. This presentation demonstrates our use of the 1,8-naphthalimide as an excellent chromophore which can be functionalised to enable LD imaging capacity, due to its large Stokes shifts, excellent photostability and high quantum yields. We have developed a series of 3,4-dialkoxy-1,8-naphthalimides and evaluated their cellular uptake behaviour. From this series, DMN-LD was identified as a selective and non-toxic LD imaging agent at low concentrations (2 µM), which can be used in both live and fixed cells. More recently we have developed an imaging agent (DMN-DT) capable of staining LD and endoplasmic reticulum regions of live cells, and consequently able to capture LD biogenesis in live cells. These probes, along with several other derivatives currently in the validation stage, permit visualisation of critical LD behaviours including homotypic fusion events, interactions with other cellular compartments, and have been used to assess the effects of drugs on lipid metabolism in live cells. These probes provide cell biologists useful tools to better investigate altered lipid biology in disease states.