Bacterial cell poles are distinctive membrane domains characterised by negative curvature and high cardiolipin content, serving as sites of intense metabolic activity. Membrane disruptors, a class of antimicrobials, can be structurally modified to interact with this domain, permitting the exploration of the subsequent metabolic shift. Here we show the redistribution of sulphur to the bacterial poles as consequence of membrane perturbation; a phenomenon we term polar sulphur enrichment. Using correlative ion probe imaging, we captured polar sulphur enrichment in both Gram-positive and Gram-negative bacteria, with increasing polar to lateral signal ratios following treatment. The ability of discrete amphiphilic dendron architectures to rapidly accumulate at the poles deemed to initiate polar sulphur enrichment with slower structural variants lacking this effect. The successive metabolic profiling revealed depletion of purine nucleotides and collapse of central energy cycle surpassing adaptive stress responses. We propose polar sulphur enrichment as elemental signature of oxidised, aggregated proteins accumulated at bacterial cell poles through nucleoid occlusion following membrane damage. These findings establish bacterial poles as targetable domains, identifiable by polar sulphur enrichment marking acute membrane stress.