The development of new methods for assessing food quality and safety is essential to protecting public health. While conventional instrument-based techniques, such as chromatography and mass spectrometry, are widely used, luminescence analysis has emerged as a powerful alternative due to its high sensitivity, selectivity, and non-destructive nature. In this context, responsive probes (sensors) have gained increasing attention for their ability to detect target analytes with precision and reliability. My research group focuses on the development of responsive probe technologies for bioassays [1]. We recently applied the probes for the luminescence analysis of food samples to ensure food quality and safety. The responsive probes with unique luminescence properties can specifically and sensitively respond to specific analytes and show changes in luminescence signals that can be recorded and quantified [1]. We have applied these probes to detect residues [2], nutrition [3], toxins, and pollutants in various food matrices, including meat, wine, dried and fresh fruits and milk. Furthermore, we have also developed portable test strips incorporating responsive probes, enabling rapid and user-friendly, onsite food testing where traditional laboratory facilities may not be accessible. Our findings aim to contribute to the advancement of next-generation analytical tools that help safeguard the food we consume. In this presentation, I will share an overview of our research and highlight the potential of responsive luminescent probes to improve the sensitivity, selectivity, and accessibility of food quality and safety monitoring.